The colon length of each mouse was individually measured (from the cecum to rectum), and the values obtained were used to indicate the mean of each experimental group, in cm. Values are expressed in mean standard deviation. Lollo P. C. B., Cruz A. G., Morato P. N., Moura C. S., Carvalho-Silva L. B., Oliveira C. A. F., et al.. (2012). For that, 96-well plates (Nunc-Immuno Plates, MaxiSorp) were coated with anti-IgA antibodies (Southern Biotechnology, Birmingham, AL, United States) and incubated overnight. Frescal cheese (as the name implies) is served quite fresh, about 4-10 days after preparation, still white and tender. PDF Life cycle assessment of minas frescal cheese and cured minas cheese: a The -amylase inhibitory activity was measured, and 100 l of human salivary -amylase (20 units/ml) with 100 l of water-soluble extracts was added and incubated at 37C for 20 min. Thus, it was necessary to investigate whether the Minas Frescal cheese manufactured with L. lactis NCDO 2118 altered the centesimal composition and bioactive compounds of the cheese, as well as whether Minas Frescal cheese was a good matrix to maintain the viability of this probiotic strain. https://www.medicalnewstoday.com/articles/150322.php. The hosts cytokine-mediated immune response plays a pivotal role during the development of acute colitis (Ko and Auyeung, 2014). The UC disease symptoms include weight loss, tummy pain, recurring diarrhea with blood in the stool, and malaise (Zhang and Li, 2014). On the other hand, we observed an increase in the expression of iNOS and IL-1 genes, when compared with the DSS group and naive group (p < 0.0001). 352 of September 4, 1997. Prior to intragastric gavage, cheese samples were daily prepared resuspending 250 mg of each cheese, separately in 250 ml of phosphate buffer (pH 7.4; 1:1), and homogenized for 2 min using an IKA T 10 Basic Ultra Turrax homogenizer. Silva H., Balthazar C. F., Esmerino E. A., Vieira A. H., Cappato L. P., Neto R. P. C., et al.. (2017). Esterhzy D., Canesso M. C. C., Mesin L., Muller P. A., de Castro T. B. R., Lockhart A., et al.. (2019). Then, the sections were photographed (20 magnification objective) using a digital camera (Spot Insight Color) coupled to an optical microscope (Olympus, BX-41, Japan). However, IL-10 staining in regulatory T cells populations can be conducted to elucidate this hypothesis. (2020). sobre a microbiota intestinal de leites. These minerals help support bone strength, cell function, muscle and tissue repair, and more. Regarding lactose amount, conventional cheese shows 2.2 0.3, while probiotic cheese presented 1.8 0.1 g/100 g. Regarding Na and Ca values, probiotic Minas Frescal cheese exhibited 549 and 316 mg/kg, respectively; meanwhile, conventional cheese presented similar values, 542 mg/kg of sodium and 312 mg/kg of calcium. Braz J Pharm Sci 42:116, CAS Publication types Research Support, Non-U.S. Gov't MeSH terms Animals Brazil Cattle Asterisks represent statistically significant differences, as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Matera J., Luna A. S., Batista D. B., Pimentel T. C., Moraes J., Kamimura B. Cordeiro B. F., Oliveira E. R., da Silva S. H., Savassi B. M., Acurcio L. B., Lemos L., et al.. (2018). UC treatments are based on the control of the symptoms and administration of anti-inflammatories and antibiotics, immunosuppressive drugs, and surgeries in severe cases. Paneer is made using either buffalo milk or cow milk. Tong L., Hao H., Zhang X., Zhang Z., Lv Y., Zhang L., et al.. (2020). Our results showed that mice in the DSS group had an inflammatory infiltrate with a very high level of neutrophils (0.0248, 0.008, p < 0.001) when compared with the naive group (0.0109 0.003). Food Res Int 76:611617, Back DMP, Andrade D, Simes G, Richards N (2013) Probiotic viability of Minas Fresh cheeses with reduced lactose content. EF performed, analyzed, and interpreted the histological analysis from colon slides. iNOS genes analyzed by qRT-PCR. Combining selected immunomodulatory Propionibacterium freudenreichii and Lactobacillus delbrueckii strains: reverse engineering development of an anti-inflammatory cheese. Apply for Overnight Specialty Team Member (Wine, Beer, Cheese, Chocolate)-Full Time job with Whole Foods in Denver, Colorado, United States of America. This suggests that the effect of probiotic cheese may be associated with a decrease of pro-inflammatory Th1 and Th17 cytokines that can be linked to the enhanced production of IL-10 in the lymph nodes and spleen, as previously reported (Santos Rocha et al., 2014). Sensory acceptance and physico-chemical composition of mixed minas Lactobacillus rhamnosus EM1107 in goat milk matrix modulates intestinal inflammation involving NF-B p65 and SOCs-1 in an acid-induced colitis model. The -glucosidase inhibitory activity was determined dissolving 100 l of -glucosidase (0.2 units/ml) in 100 l of phosphate buffer (pH 6.8), mixed with 150 l of water-soluble extracts, and incubated for 20 min at 37C. Thus, both kinds of cheese did not alter the daily caloric content. Mucins in intestinal mucosal defense and inflammation: learning from clinical and experimental studies. zo-1, (B) They were housed in plastic cages in a room with controlled temperature (1823C), light cycle of 14-h light/10-h dark, relative humidity (4060%), and ad libitum access to food and water. For MPO quantification, a piece of colon tissue (10 mg) was homogenized proportionally in 1.9 ml/100 mg of PBS and centrifuged at 12,000 g for 10 min. The supernatant was discarded, and the pellet formed was lysed and centrifuged again. Clin Infect Dis 31:10121017, Arvola T, Laiho K, Torkkeli S, Mykkanen H, Salminen S, Maunula L, Isolauri E (1999) Prophylactic Lactobacillus GG reduces antibiotic-associated diarrhea in children with respiratory infections: a randomized study. (2020). Is cottage cheese good for you? Plus how it compares to yogurt. - USA TODAY Inflammatory bowel diseases (IBDs) constitute disturbances of gastrointestinal tract that cause irreversible changes in the structure and function of tissues. Rev Bras Nutr Clin 27:264272, Bartran HP, Sheppach W, Schmid H, Hofmann A, Dusel G, Richter F, Richter A, Kasper H (1993) Proliferation of human colonic as an intermediate biomarker of carcinogenesis: effects of butyrate, deoxycholate, calcium, ammonia, and pH. Official Union Journal. Data were analyzed using one-way ANOVA followed by Tukey post-test and performed in GraphPad Prism version 7.00 for Windows (GraphPad Software, San Diego, CA, United States). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). Clinicopathologic study of dextran sulfate sodium experimental murine colitis. In: Tannock GW (ed) Probiotics: a critical review. Food Chem 196:628637, Talwalkar A, Miller CW, Kailasapathy KN, Guyen MH (2004) Effect of packaging materials and dissolved oxygen on the survival of probiotic bacteria in yoghurt. Some of these peptides can resist gastrointestinal digestion, responsible for biological activities such as antihypertensive, antioxidant, and antidiabetic activities (Livney, 2010). (PDF) Microbiological quality of Minas Frescal cheese treated with Moreover, there was an increase in inflammatory cell infiltration in the lamina propria, i.e., neutrophil infiltrates, and also depletion of goblet cells (Jeengar et al., 2017). Figure 1E shows that treatment with probiotic cheese has a protective effect on colitis-induced body weight loss. Rev Inst Lat Cnd Tostes 68:2735, Paula JCJ, Carvalho AF, Furtado MM (2009) Princpios Bsicos de Fabricao de queijo: Do Histrico Salga. official website and that any information you provide is encrypted We evaluated the performance of check-all-that-apply (CATA) questions and intensity scales to describe Minas Frescal cheese and its reformulation based on consumers' perceptions. Porto B. Crdoba, FAO/WHO Expert Consultation, Dantas AB, Jesus VF, Silva R, Almada CN, Esmerino EA, Cappato LP, Silva MC, Raices RSL, Cavalcanti RL, Carvalho CC, SantAna AS, Bolini HMA, Freitas MQ, Cruz AG (2016) Manufacture of probiotic Minas Frescal cheese with Lactobacillus casei Zhang. After incubation at 37C for 20 min, the reaction was stopped, and 80 l of sodium carbonate solution (0.2 mol/l) was added. CRC Press, Boca Raton, pp 1369, Lourens-Hattingh A, Viljoen BC (2001) Yogurt as probiotic carrier food. Carvalho R. D., do Carmo F. L. R., de Oliveira Junior A., Langella P., Chatel J. Mouse body weight was individually measured during all experimental days. Our results demonstrated that the antioxidant potential (DPPH), ACE inhibitory activity (ACEI), -amylase, and -glucosidase on the probiotic cheese, containing L. lactis NCDO 2118, presented increased values and were significantly different (p < 0.05) compared with conventional cheese. The data represent the mean SD (n = 6). Physicochemical, nutritional, and sensory attributes of Minas frescal The proximate composition (moisture, protein, and fat) was evaluated according to the methodology previously described (BRASIL, 2006). In this context, functional probiotic foods have been suggested to be used alone or in combination with conventional drugs and act like adjuvant therapy to enhance remission in UC patients (Rabah et al., 2020). Quantitative PCR (qPCR) was determined using iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, United States) and gene-specific primers, according to Do Carmo et al. Konrad B., Anna D., Marek S., Marta P., Aleksandra Z., Jzefa C. (2014). Our work suggests that Foxp3+ Tregs are not responsible directly for the therapeutic effects of probiotic bacteria, despite increased levels of IL-10 (lymph nodes and spleen). Values indicate the mean standard deviation. Besides that, we observed that probiotic cheese was able to decrease the expression of iNOS gene. In this work, we observed that unhealthy mice treated with probiotic Minas Frescal cheese exhibited attenuated clinical and macroscopic signs of colitis disease. In this way, this work demonstrates that consumption of probiotic Minas Frescal cheese, containing L. lactis NCDO 2118, prevents the inflammatory process during DSS-induced colitis in mice, opening perspectives for the development of new probiotic functional foods for personalized nutrition in the context of IBD. Intech, Rijeja, pp 129148, Ribeiro EP, Simes LG, Jurkiewicz CH (2009) Desenvolvimento de queijo minas frescal adicionado de Lactobacillus acidophilus produzido a partir de retentados de ultrafiltrao. Then, 0.2 g/l of calcium chloride (Labsynth, Sao Paulo, Brazil) and 3 g/l of coagulant powder (Halamix power, Chr. Functional foods containing probiotic bacteria have been studied as adjuvants to the treatment or prevention of IBDs. 5. Design/methodology/approach Noteworthy, the Minas Frescal cheese is one of the most consumed cheese in Brazil and constitutes one important activity of the dairy industries, due to the high yield and absence of maturation period, which allows a quick return on investment and, consequently, lowers costs to the consumer ( Sperry et al., 2018; Rocha et al., 2020 ). Grom L. C., Rocha R. S., Balthazar C. F., Guimares J. T., Coutinho N. M., Barros C. P., et al.. (2020). However, the addition of probiotic cultures can have a negative impact on the physicochemical and sensory characteristics of the products. The frontier between nutrition and pharma: the international regulatory framework of functional foods, food supplements and nutraceuticals. However, when mice were treated with probiotic cheese, we found a significant reduction of these cells (0.0137, 0.003, p < 0.05), showing MPO levels very similar to those found in healthy animals (p > 0.05). The administration of DSS provokes a substantial decrease in the number of goblet cells in the colon tissue (56.4 25 goblet cell/hpf) when compared with the naive group (103 27.5, p < 0.01, Figures 3A,,B).B). Relationship between number of bacteria and their probiotic effects. Maldonado Galdeano C., Cazorla S. I., Lemme Dumit J. M., Vlez E., Perdign G. (2019). Ann Nutr Metab 46:34, Bu LN, Chang MH, Ni YH, Chen HL, Cheng CC (2007) Lactobacillus casei rhamnosus Lcr35 in children with chronic constipation. Effect of probiotic cheese administration on the dextran sodium sulfate (DSS)-induced histopathological damages. To measure the level of histological inflammation in the colon tissue, the score previously described was used (Wirtz et al., 2017). The intake of DSS led to increased cytokine IL-1 in the spleen (243.7 pg./ml, Figure 5E) and IL-17 in the lymph nodes (163.9 pg./ml, Figure 5I), when compared with the naive group (123.8 and 44.4 pg./ml). Abstract The buffalo Minas Frescal cheese with Bifidobacterium BB-12 and the MRS broth, as a control, were used under in vitro simulated gastrointestinal conditions. IL-17 genes analyzed by qRT-PCR. being able to inactivate vegetative and sporulated The microbiological parameters of this cheese cells, yeast, molds and viruses, additionally is able are established by Brazilian law (Brasil, 1996; Brasil, to kill pests of grain storage (ins. On the other hand, to uncover the exact mechanisms, the expression of intestinal immune-related gene and sIgA levels needs to be better explored. Neurology 87:12741280, Dimidi E, Christodoulides S, Scott M, Whelan K (2017) Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation. Interestingly, it is recognized that oxidative stress (OS) is one of the factors involved in the onset of IBD symptoms (Moura et al., 2015); therefore, we suggest that the increase in DPPH levels in probiotic Minas Frescal Cheese could help to ameliorate inflammation conditions in UC mice. Body weight variation was also statistically different (p < 0.05) between DSS + conventional cheese (4.5 5.9%) and DSS + NCDO cheese (+0.23 0.80%). FOIA Experimental groups and the respective treatments. LWT Food Sci Technol 63:11791183, Fritzen-Freire CB, Mller CMO, Laurindo JB, Prudncio ES (2010) The influence of Bifidobacterium Bb-12 and lactic acid incorporation on the properties of Minas Frescal cheese. Save some prep time by swapping out the fresh herbs and use a rubbed Fontal, like the smoky pepper wedge or the fragrant bergamot and hibiscus. Best Pract Res Clin Gastroenterol 18:299313, Puupponen-Pimi R, Aura AM, Oksmancaldentey KM, Myllrinen P, Saarela M, Mattila-Sanholm T, Poutanen K (2002) Development of functional ingredients for gut health. Therapeutic Effects of Probiotic Minas Frescal Cheese on the The quantitative gene expression in colon fragment was determined according to Do Carmo et al. To obtain the pH levels of both cheese, we inserted a digital pH meter electrode (Micronal, B-375, Digimed, Piracicaba, So Paulo, Brazil) into the diluted cheese samples as previously described (Silva et al., 2017). Cinc Tecnol Alim 29:1923, Alves CCC, Gemal NDH, Cortez MAS, Franco RM, Mano SB (2011) Lactobacillus acidophilus and of direct acidification to Minas Frescal cheese production. ab Different letters in the same row indicate a significant difference (p < 0.05). Acute colitis induced by dextran sulfate sodium progresses to chronicity in C57BL/6 but not in BALB/c mice: correlation between symptoms and inflammation. This work was supported by the Fundao de Amparo Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico (CNPq), and Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES). Chemically induced mouse models of acute and chronic intestinal inflammation. The amplification cycles were performed as follows: 95C for 30 s and 40 cycles of 95C for 15 s and 60C for 30 s on ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). However, there are non-dairy cream alternatives, such as cashew cream and coconut cream. Bacterial viability in both cheese solutions was determined by CFU counts. (2018) suggested that Lactobacillus casei 01 can generate high levels of antihypertensive (ACE-I) and antioxidant peptides (DPPH) in Minas Frescal cheese. A., Monteiro C. F., Souza . L. S., Leocdio P. C. L., Alvarez-Leite J. I., Generoso S. V., et al.. (2019). Effect of Lactobacillus rhamnosus on growth of Listeria monocytogenes To realize the enzymatic assay, we added an equal amount of substrate (1.5 mM/l of OPD and 6.6 mM/L of H2O2 in 0.075 mM/L of Tris-HCl pH 8.0) to the supernatant. This cheese type is classified as semi-fat, non-ripened, low salt, pH greater than 5.0, and high humidity, which is obtained by enzymatic coagulation of milk with rennet (Felicio et al., 2015). The probiotic cheese, containing L. lactis NCDO 2118, did not change the percentage of Tregs on the spleen and lymph nodes in both healthy and inflamed mice. MUC-2, and (F) Ma J., Lorn V., Pedrosa E., Ojanguren I., Xaus J., Cabr E., et al.. (2009). (2022), fermented cheeses. For stop reaction, 20 l/well of 2 N of H2SO4 was added. 6 Citations Metrics Abstract We aimed to evaluate the chemical composition, production yield, and economic viability of Minas Frescal cheese produced with milk from buffaloes supplemented with aai seed as a substitute for corn meal.
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